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siRNA enrichment in Argonaute-2 depleted Blattella germanica

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP300141
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Using as a model the German cockroach, Blattella germanica, we examined the activity of AGO2 by depleting its mRNA. A second treatment with a dsPolyh (and heterologous dsRNA) allows the analysis of the siRNAs generated from this dsRNA, in control and AGO2-depleted animals. The use of a heterologous dsRNA (dsPolyh) allowed us to dissociate the activity of dsRNA processing and selection of the guide siRNA from the action of cleavage a complementary RNA.
创建时间:
2022-01-01
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