Direct Induction of Trophoblast Stem Cells from Human Fibroblasts (RRBS). Direct Induction of Trophoblast Stem Cells from Human Fibroblasts (RRBS)

Transcription profile analysis revealed similarity/dissimilarity between human blastocyst derived trophoblast stem cells (hbdTSCs), human induced trophoblast stem cells (hiTSCs), human foreskin fibroblasts (HFFs) and pluripotent stem cells (hESCs, hiPSCs) Overall design: HFFs reprogrammed into hiTSC lines by transient ectopic expression of GATA3, OCT4, KLF4, MYC (GOKM) were subjected to RRBS-protocol to assess differentially methylated regions, along with samples of HFFs, hbdTSCs, hESCs and hiPSCs