WGS of BL21(DE3) derivative Evo21(DE3)

Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicityGene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Here we evolve Escherichia coli BL21(DE3) - the most popular choice for recombinant protein production - to facilitate improved yield and tolerance to toxic genes isolating derivative Evo21(DE3) and pin point a key role for RNA stability in gene expression toxicity.