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2022-10-26
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A Modular Scaffold for Controlling Transcriptional Activation via Homomeric Protein–Protein Interactions
Protein–protein interactions (PPIs) have been extensively utilized in synthetic biology to construct artificial gene networks. However, synthetic regulation of gene expression by PPIs in E. coli has l
Figshare80
Additional file 2: of GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris
GoldenPiCS modules and plasmids. Modules and plasmids are listed with corresponding cloning- and fusion sites and full sequences. DNA orientation is 5’to 3′. All plasmids are available at Addgene. (XL
Figshare2017-12-09 更新60
EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology
Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly of plasmid constructs often used in combinatorial pathway optimization, with a number of assembly kits develo
NIAID Data Ecosystem30
Bacterial strains and plasmids used in the study.
*Sm, streptomycin; Cm, chloramphenicol; Kan, kanamycin; Amp, ampicillin.
NIAID Data Ecosystem60



