Gene expression changes in REH following STAT3 pharmacological inhibition. Gene expression changes in REH following STAT3 pharmacological inhibition

STAT3 (Signal transducer and activator of transcription 3) expression is associated with with t(12;21) acute lymphoblastic leukaemia (ALL) and it is crucial for the survival of the t(12;21) ALL . Our study investigated the STAT3 regulated pathways and discovered a novel STAT3-TP53 axis in B-ALL. In order to determine the STAT3 regulated pathways in t(12;21) ALL cells, we performed RNAseq analysis on the Pre-B Acute Lymphoblastic Leukemia (ALL) cell line REH, treated with DMSO vehicle control in comparison to 50uM S3I-021, a specific STAT3 inhibitor Overall design: Total cellular RNA was purified from DMSO and 50uM S3I-201 treated REH samples from three independent experiments