Deep mutational scanning of Trypanosoma brucei RBP6.

This BioProject contains 50 files with paired-end reads of 25 libraries derived from the Deep Mutational Scanning of T. brucei RBP6. Briefly, mutant plasmid libraries (PL), containing most single-point variants of RBP6, were stably transfected in procyclic T. brucei forms generating mutant starting libraries (ST). Expression of mutant variants was induced for 6 or 3 days and input (IN) populations were used to purify metacyclic forms (MC). Wild-type (WT) libraries were subjected to the same experimental conditions as controls. RBP6 variants were sequenced from plasmids or genomic DNA from ST, IN, and MC cells using a barcoded-subamplicon sequencing strategy (https://jbloomlab.github.io/dms_tools2/bcsubamp.html). One or three replicates (Rep1-3) were obtained for each WT or mutant libraries, respectively.