Exp_20211226_1_BH_RI_batch_wc

Growth kinetics of monocultures Blautia hydrogenotrophica S5a33 (DSM 10507T) and Roseburia intestinalis L1-82 (DSM 14610T) and their co-culture in Wilkins-Chalgren Anaerobe Broth. Notes: live/dead cell staining followed by flow cytometry, using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA); anaerobic cultivation of 120 h for the experiment In addition to the instrumental calibration with CytoFLEX Daily QC Fluorospheres, 0.5 μm and 1 μm green-fluorescent beads (Thermo Fisher Scientific, USA) were applied to each batch of experiments as internal standards, to monitor the instrument stability and to make cytometric data from different batches more comparable.