sRNA-seq of Aspergillus fumigatus RNAi double knockouts expressing a pksP inverted-repeat transgene

The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be altogether lost to improve virulence potential in some fungal pathogens. In the WHO fungal priority pathogen, Aspergillus fumigatus, the RNAi system is known to be intact and functional. To extend our limited understanding of A. fumigatus RNAi, we performed a sRNA-seq to assess sRNAs produced in several RNAi double knockouts and a wild type strain expressing a 1200 nt inverted-repeat transgene with complementarity to the pksP gene. The transgene harbors a 500-bp inverted repeat. We included an uninduced wild type strain with no inverted-repeat transgene as a control. All conditions were performed in mycelium grown for 24 hours in liquid culture. Overall design: Small RNA profiling analysis of Aspergillus fumigatus RNAi double knockouts (??dclA/B, ??ppdA/B, ??rrpA/B) and wild type expressing a 1200 nt inverted-repeat transgene with complementarity to the pksP gene, using 24-h-mycelium. Please note that GSM8721378-GSM8721380 raw files and IRT_sRNA_processed.csv processed data file have been replaced on Aug 11, 2025.