Expression data from rostral forebrains of wild-type and Fezf1-/- Fezf2-/- mice

Zinc-finger genes Fezf1 and Fezf2 encode transcriptional repressors. Fezf1 and Fezf2 are expressed in the early neural stem/progenitor cells and control neuronal differentiation in mouse dorsal telencephalon. We compared gene expression profiles of rostral forebrains, which contain the telencephalon and the rostral part of the diencephalon, from embryonic day (E) 9.5, E10.5, and E12.5 wild-type control and Fezf1-/- Fezf2 -/- mouse embryos. The forebrain rostral to the caudal limit of the lateral ventricles was isolated manually from E9.5, E10.5, and E12.5 wild-type and Fezf1-/- Fezf2-/- mice. Total RNAs were isolated by Separsol-RNA I and were used for microarray analyses.锌指基因Fezf1与Fezf2编码转录抑制因子。Fezf1与Fezf2在早期神经干细胞/祖细胞中表达，并调控小鼠背侧端脑的神经元分化进程。本研究针对胚胎第9.5天（E9.5）、E10.5及E12.5的野生型对照与Fezf1-/- Fezf2-/-小鼠胚胎的嘴侧前脑——该组织由端脑与间脑嘴侧部分构成——的基因表达谱开展了比较分析。研究人员手工分离了上述三个发育时点的野生型与Fezf1-/- Fezf2-/-小鼠中，位于侧脑室尾侧界限嘴侧的前脑组织。采用Separsol-RNA I试剂提取总RNA，随后将其用于基因芯片分析。