PFIbox Protocol Datasets

Data accompanying the Nature Protocols manuscript entitled "A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library". This dataset contains gene expression data from E. coli grown in MOPS-glucose medium over 24 hours, sampling at 5 minute intervals. This also contains the data for Figure 3, 9, and 10 for this manuscript: Figure 3 is cumulative gene expression in MOPS-glucose after 24 hours of growth; Figure 9 are clusters of co-expression after using fuzzy c-means clustering to look at gene expression profiles; Figure 10 is the fold change between MOPS-glucose and lysogeny broth (LB) media, which is provided as promoter, -log10 of P-value, and log2 of fold-change (n=3).