Next Generation Sequencing Facilitates Quantitative Analysis of control and MILIP Knockdown by siRNA in A549 human lung cancer cell line

Enriched p53 signalling after MILIP knockdown was validated by RNA-seq; A c-myc reponsive lncRNA MILIP is commonly upregulated in diverse cancer types. RNA-seq analysis of A549 cells following MILIP knockdown revealed that p53 signalling was the mostly enriched gene pathway, suggesting c-Myc may inactivate p53 through MILIP in cancer. Overall design: For RNA-seq analysis of MILIP siRNA versus control siRNA, the A549 cells were transfected with siRNAs for 48 hours before harvesting.