Alteration of Gene Expression Profile in Spontaneously Hypertensive Rats Treated with Protein Hydrolysate of Blue Mussel (Mytilus edulis) by DNA Microarray Analysis

The aim of this study was to investigate the antihypertensive effect of enzymatic hydrolysis of blue mussel protein (HBMP) in rats. Spontaneously hypertensive rats (SHRs) were orally administration with high- or low-dose of HBMP for 28 days. Major components of the renin-angiotensin (RAS) system in serum of SHRs from different groups were analyzed, and gene expression profiling were performed in the kidney of SHRs, using the Whole Rat Genome Oligonucleotide Microarray. Results indicated although genes involved in RAS system were not significantly altered, those related to blood coagulation system, cytokine and growth factor, and fatty acids metabolism were remarkablely changed. Several genes which were seldom reported to be implicated in pathogenesis of hypertension also showed significant expression alterations after oral administration of HBMP.本研究旨在探究蓝贻贝蛋白酶解产物（HBMP）对大鼠的抗高血压效应。将自发性高血压大鼠（SHRs）分为高、低剂量给药组，经口给予HBMP并持续28天。对不同给药组SHRs血清中的肾素-血管紧张素（RAS）系统主要组分进行分析，并采用全大鼠基因组寡核苷酸微阵列对SHRs肾脏组织进行基因表达谱检测。结果表明，尽管RAS系统相关基因未出现显著表达变化，但与凝血系统、细胞因子与生长因子以及脂肪酸代谢相关的基因均发生了显著改变。此外，少数此前鲜有报道参与高血压发病机制的基因，在经口给予HBMP后也呈现出显著的表达差异。