Poly(A) mRNA-seq from yw and Twi-mCherry-LEXY(V416L) embryos subjected to different blue light exposure schemes

Profiling of genes expression changes caused by the conditional perturbation of Twist function during different developmental time windows of Drosophila embryogenesis Twist was depleted from the nucleus continuously or post-gastrulation using the improved blue light-inducible mislocalisation system LEXY(slow). Gene expression changes between dark- and blue light-incubated embryos were profiled 2-4 h and 4-6 h after egg lay. yw embryos serve as a wild-type control. Poly(A) mRNA-seq was performed from three biological replicates for Twi-mCherry-LEXY(V416L) embryos and two biological replicates for yw embryos.