Polystyrene-induced changes in adipose transcriptome

Microplastics (MP) derived from the weathering of polymers, or synthesized in this size range, have become widespread environmental contaminants and have found their way into water supplies and the food chain. Despite this awareness, little is known about the health consequences of MP ingestion. We have previously shown that the consumption of polystyrene (PS) beads was associated with intestinal dysbiosis, diabetes, and obesity in mice. To further evaluate the systemic metabolic effects of PS on the gut-liver-adipose tissue axis, we supplied C57BL/6J mice with normal water or that containing two sizes of PS beads (0.5mm and 5mm) at a concentration of 1mg/ml. After 13wk, we evaluated several indices of metabolism and liver function. As previously observed, mice drinking the PS-containing water had a potentiated weight gain and adipose expansion. Here we found that this was associated with an increased abundance of adipose F4/80+ macrophages. These exposures did not cause non-alcoholic f..., To perform the adipose transcriptomic analysis, RNA was prepared from tissue sections using a miRNeasy kit (Qiagen). Concentrations were determined using a NanoDrop instrument (Thermo Scientific). RNAseq and data analysis utilizing the Kyoto Encyclopedia of Genes and Genomes (KEGG, Gene Ontology classifications (GO), and the Reactome Database was performed by Novogene., , # adipose RNA seq data

RNAseq data from epidydimal white adipose tissue isolated from 2 mouse treatment groups:
group1-mice consuming water containing 0.5 micron polystyrene beads and injected with DMSO
group2- mice consuming water containing 0.5 micron polystyrene beads and injected with delphinidin

## Description of the data and file structure

Two Excel files listing the differentially expressed genes (DEGs) are supplied. One file lists those genes which are up-regulated in the delphinidin group versus the DMSO group (TO-DELPvsDMSO_up.xlsx). The second file lists those genes which are down-regulated in the delphinidin group versus the DMSO group (TO-DELPvsDMSO_down.xlsx). In each Excel file, there are two tabs listing the DEGs. One tab lists in order of fold change, while the other lists in order of p-value. As columns in each tab and in each file, there are: A: level in the delphinidin group; B: level in the DMSO group; C:log2 fold change; D: p value; E: adjusted p value; F: gen...