Transcription profiling by array of mouse hepatocytes following treatment with growth factor beta 1 in a time series. Transcription profiling by array of mouse hepatocytes following treatment with growth factor beta 1 in a time series

To obtain a comprehensive view on the TGF-beta effects in primary mouse hepatocytes, Affymetrix microarrays were performed. We aimed at detecting early (1h), intermediate (6h) and late (24h and 48h) target genes. Overall design: Primary hepatocytes were used as an in vitro model to detect TGF-beta effects on the expression profile. In primary cell cultures, the majority of hepatocytes survive despite activated TGF-beta signaling. These cells display phenotypic characteristics for (partial) EMT and fibrogenesis.