RNA seq analysis of Gpr56/Adgrg1 expressing 4T1 and TS/A cells cultured with type III collagen

We founed enhanced Gpr56/Adgrg1 mRNA expression in bone tropic mouse breast cancer cell lines, 4T1.3, grown in a bone cavity, compared with in vitro culture condition. Thus, we examined about the function of Gpr56/Adgrg1 in tumor growth of vitro culture condition with its ligand, type III collagen. Overall design: Mouse breast cancer cell lines 4T1 and TS/A cells which were transduced Gpr56/Adgrg1 gene were cultured with type III collagen. After 72H, total RNAs was extracted. The resultant total RNAs were subjected to RNAseq.