Location, genomic and protein effect of predicted SNPs by NGS and primers for validation by Sanger sequenzing.
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PCR conditions: 10μl HF buffer, 1μl dNTPs, 0.5μl phusion polymerase; all Phusion High fidelity Kit; 2.5μl forward and reverse primer (BiomersGmbH, Ulm, Germany), 100ng sample DNA and nuclease free water adding up to a final volume of 50μl; 98°C 1 min, 32 cycles of 98°C 5 sec/ 68°C 20 sec / 72°C 25 sec, 72°C 10 min. Nonsyn. SNV = nonsynonymous single nucleotide variant. (DOCX)
创建时间：
2015-04-02
