PCRs used in the present study with target sequence, primer name and sequences, length of expected amplicon, reaction mixtures and cycling conditions.
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Reaction mixture 1: 25 μl containing 25 ng DNA, 1X CoralLoad buffer, 1.5 mM of MgCl2, 200 μM of dNTPs, 0.5 μM of each primer, 0.5 U of HotStar TaqPlus. Reaction mixture 2: 25 μl containing 25 ng DNA, 1X CoralLoad buffer, 1.5 mM of MgCl2, 200 μM of dNTPs, 1 μM of each primer, 0.5 U of HotStar TaqPlus. Reaction mixture 3: 25 μl containing 25 ng DNA, 1X CloneAmp HiFi PCR premix and 0.25 μM of each primer. bp: base pair, P: Plus DNA strand, M = Minus DNA strand.
创建时间：
2016-04-04
