Transcriptome analysis on micro-tissue samples collected from Arabidopsis thaliana cotyledons

We investigated how the shade stimulus is spatially processed within the cotyledon in plants. For this purpose, we performed transcriptome analysis on micro-tissue samples collected from vascular and non-vascular regions of Arabidopsis thaliana cotyledons and subjected to transcriptome analysis by RNA sequencing. In addition, we mechanically isolated and analyzed the vascular tissue in a similar manner.