RNA-seq of B16F10 cancer cells treated with different conditioned media from cancer cell-induced TAM

We analyzed the transcriptomes of B16F10 cells treated with different conditioned media from BMDM, M1-BMDM, M2-BMDM or B16F10-induced TAM. Overall design: BMDM cells were treated by control media (DMEM), 20ng/ml INF-?,20ng/ml IL-4, or 75% B16F10 conditioned media (CM) for 24 h to obtain M0-BMDM CM, M1-BMDM CM, M2-BMDM CM and B16F10-BMDM CM respectively. Then different CM from BMDM was used to treat B16F10 cells for another 24 h. Then the RNA of B16F10 cells was extracted and underwent RNA-seq.