FigS2_RawData

Figure S2. µXRF maps of element distributions in unstained methacrylate-embedded thin mid-tubule sections of woodlouse hepatopancreas sampled at 02:00 hours (i.e. the beginning of ‘B’-cell restitution during darkness). A. Light micrograph of a transverse section. S5_sp_light1x10.tif B. Superimposed Cu, Fe and Zn µXRF maps of the section depicted in S1A; note the regular 1:1 distribution of ‘S’- (*) and ‘B’-cells (•) in the tubule wall. Prefix 5955 C. Expanded view µXRF maps for the region delineated by a broken-lined rectangle in 2E; note the hallmarks of Cu/Zn-containing ‘S’-cell cytoplasm ‘under’ the basal aspect of the ‘B’-cell (dotted line), the co-distribution of Cu and Zn (light blue) as well as partitioning of Cu (green) and Zn (dark blue) in the ‘S’-cell, and possibly some Zn-containing focal regions in the ‘B’-cell (broken arrows). Prefix 5956 File types included: tif - Image files (Light Micrographs) .dat : map data showing the stage positions in mm and windowed fluorescence counts. Column format: Horizontal position ; vertical position; time per point (ms); I0; It; Idrain; mca file. .edf file ESRF format file for program PyMCA containing the summed MCA files for each point in a map row by row. Binary data format .rgb file windowed detector counts for selected chemical elements of interest , column format: y index; x index; time; i0 it idrain; then elements as listed in first row.