Polymorphisms within regions of PfRh4.

Notes: Sequence analysis of PfRh4.2 and PfRh4.9 in clinical isolates derived from the Mugil population (I), global sequences of diverse geographic origins (II) and the combined datasets (III). For global sequences, we sequenced isolate HCS3, and obtained sequences for 3D7, Dd2, HB3, 7G8, FVO, CS2, D6, FCC2, IGH-CR14, K1, Pf_Senegal, SL and VS/1, IT line and Ghana from the Broad Institute and Sanger Institute public databases. N: number of isolates tested. bp: length of fragment sequenced in bp (PfRh4.2: bp 3829–4353; PfRh4.9: bp 170–1987). *: length of fragment varied between 1648bp and 1817 bp. SNPs: total number of single nucleotide polymorphisms observed in all sequences. h: total number of haplotypes observed. S: total number of synonymous mutations in all sequences. NS: total number of non-synonymous mutations in all sequences.