Imaging Ng2:DsRed+ Murine Retina over 6 Hours in Culture

Murine retinal explant from an Ng2/Cspg4:DsRed/+ animal cultured in Dulbecco's Modified Eagle Medium (DMEM) + 5% Fetal Bovine Serum (FBS) + 1% penicillin-streptomycin at 37 degrees Celsius for 6 hours. Explants were cultured on a glass-bottom petri dish and imaged every hour on a Zeiss LSM 880 point-scanning confocal microscope. Volumetric images taken through the z-axis were acquired at 40x and 63x. Images in the figure are compressed z-stacks within a selected range, but the raw image files in .czi format are also included.