Anti-HER2/neu TCR-T Cells in Action: Linking Transcriptional Signatures, Secretomics, and In Vivo Tumor Suppression

We performed single-cell RNA sequencing using the BD Rhapsody platform to profile HER2/neu-specific TCR-engineered human T cells following antigen stimulation. TCR-T cells and non-transduced control T cells were analyzed after co-culture with HER2/neu-expressing tumor cells. Overall design: Peripheral blood CD3+ T cells from healthy HLA-A*02:01 donors were isolated and activated ex vivo. Cells were transduced with a lentiviral vector encoding a HER2/neu-specific TCR or kept non-transduced (LV-neg). After co-culture with HER2/neu-expressing tumor cells for 16–18 hours, TCR-T and LV-neg cells were stained with SampleTag and processed using the BD Rhapsody single-cell workflow.