Fructose 2,6-bisphosphate is hydrolyzed to form fructose-6-phosphate and orthophosphate

The fructose 2,6-bisphosphatase activity of cytosolic phosphofructokinase 2/fructose-2,6-bisphosphatase catalyzes the hydrolysis of fructose 2,6 bisphosphate to form fructose 6-phosphate. Fructose 2,6 bisphosphate is a key allosteric regulator of PFK1. In its absence the activity of PFK1 is reduced while fructose 1,6-bisphosphatase is activated, thus inhibiting glycolysis and favoring gluconeogenesis (Pilkis et al. 1995).

细胞质中磷酸果糖激酶2/果糖2,6-二磷酸酶的果糖2,6-二磷酸酶活性催化果糖2,6-二磷酸的水解，生成果糖6-磷酸。果糖2,6-二磷酸是磷酸果糖激酶1（PFK1）的关键变构调节因子。在果糖2,6-二磷酸缺失的情况下，PFK1的活性降低，而果糖1,6-二磷酸酶被激活，从而抑制糖酵解，促进糖异生（Pilkis等，1995年）。