Gene expression profiling induced by high dose ionizing radiation on MDA-MB-231 cells

Radiation therapy (RT) remains a vital component of the curative multimodality therapy for many types of cancer including breast cancer (BC). Even if great number of BC patients receive RT, not all of them report benefits, due to radioresistance activation depending also by hormone receptor status. We analyze and compare molecular responses, in term of gene expression profile (GEP) changes, induced by high dose of Ionizing Radiation (IR), in MDA-MB-231 with ER-/PR-HER2- receptor status. Moreover, we selected a MDA-MB-231 Radioresistent Cell Fraction (RCF), in which we highlighted specific molecules able to drive radioresistance. We trust that this study could have a role in the evaluating of RT response in term of radioresistance process activation to define a personalized biological-driven treatment plan in triple negative BC. Changes in gene expression in tumorigenic MDA-MB-231 breast cancer cell lines, exposed to 9 and 23Gy of electron irradiation, were analyzed as two-color hybridizations using Agilent Technologies whole human genome 4x44K microarrays. In addition, we have used the our existing samples following listed: GSM2916578-83; GSM3032684-89. Moreover, new samples were named as follow: MDA-MB-231_RCF rep1-rep6.