The detection and enumeration of propionibacterium spp from kefir under different storage conditions using pcr and conventional methods
收藏DataCite Commons2026-02-18 更新2026-03-29 收录
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https://esango.cput.ac.za/articles/dataset/The_detection_and_enumeration_of_propionibacterium_spp_from_kefir_under_different_storage_conditions_using_pcr_and_conventional_methods/30720059/1
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资源简介：
This study aimed to optimize a method to detect <i>Propionibacterium freudenreichii</i> in kefir during refrigerated, frozen, and freeze-dried storage for 28 days. Conventional enumeration was compared with the polymerase chain reaction (PCR) method. For culture-based analysis, serial dilutions were prepared and plated on YEL, PCA, and Pal-Propionic agar. Samples were treated with propidium monoazide before DNA was extracted using the Power Food Microbial DNA Isolation Kit. <i>Propionibacterium freudenreichii</i> was detected using PCR.
提供机构：
Cape Peninsula University of Technology
创建时间：
2026-02-18
