Survival of Salmonella enterica in the bovine lymphatics

Using massively-parallel sequencing in transposon-directed insertion-site sequencing (TraDIS) we can simultaneously assign identity and phenotype to bacterial mutants during infection. We previously assigned roles for 2721 S. Typhimurium genes in intestinal colonisation in cattle. Retrospective analysis of the same mutant library but recovered from mesenteric lymph nodes draining the terminal ileum in the same animals has identified genes with a putative role in lymphatic spread and/or survival.