Additional file 1 of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Additional file 1: Description of piggyBac insertions. Column 1: Origin of insertion described as: Parental population.clone.subclone (See main text for description). Column 2: Type of insertion in relation to CDS: 5' UTR, CDS, 3'UTR or more than 1 kb away from the nearest CDS (> 1 kb from CDS). Column 3: Insertion site distance to start ATG of CDS (for 5' UTR and CDS insertions) or from the end (stop codon) of the CDS (for 3' insertions). Column 4: Percentage of locus disrupted (for CDS insertions only) and calculated as the percentage of the locus (including introns) occurring after the insertion site. Column 5: Pb locus identifier as used by the Sanger Center (two first digits indicate chromosomal location on the 14 P. berghei chromosomes). Column 6: Insertion site sequence (TTAA) with the adjacent 20 5' and 3' nucleotides. Column 7: Gene description as provided by the Sanger Center. (XLS 46 KB)