Transcriptome analyses of mammalian tissues

In mammals, more than 60% of the genome is use for transcription for mRNAs and noncoding RNAs (ncRNAs). At present, little is known about the concerted expression of mRNAs and antisense transcripts located in their 5’-flanking regions, and the comprehensive transcriptome analysis focusing on the bidirectional transcription of mRNA and pancRNA has not been performed. We do not yet know the sequence characteristics of the bidirectionally transcribed regions. Here, we perform directional RNA sequencing in order to examine whether there is a correlation between the expression of sense and antisense RNAs at the genome wide level.