Biliary-Atresia-associated Mannosidase-1-alpha-2 gene regulates biliary and ciliary morphogenesis and laterality (mouse)

The effect of MAN1A2 on lung and liver development and ciliogenesis was evaluated with RNAseq analyses in knockout mice Total RNA was extracted from liver and lung samples from three Man1a2+/+ (WT) mice and three Man1a2-/- (Null) mice to generate mRNA sequencing libraries本研究通过对基因敲除小鼠开展RNA测序（RNAseq）分析，评估了MAN1A2对肺脏、肝脏发育及纤毛发生的影响。研究人员从3只Man1a2+/+（野生型，WT）小鼠与3只Man1a2-/-（Null型）小鼠的肝脏及肺脏组织样本中提取总RNA，以构建mRNA测序文库。