RNAseq profiling of AMCase-expressing lung epithelial cells

We have generated novel reporter mice "ChiaRed") to study the role of the acidic mammalian chitinase (AMCase) in lung homeostasis and in response to immune challenge. Lung epithelial cells from wild-type mice (WT; n=3) and AMCase-expressing ChiaRed+ cells were isolated from the lungs of homozygous (CC; n=2) and heterozygous (C+; n=3) ChiaRed mice to compare differentially expressed transcripts by RNA-Seq analysis in these cells.我们构建了新型报告基因小鼠"ChiaRed"，用于研究酸性哺乳动物几丁质酶（acidic mammalian chitinase, AMCase）在肺稳态及免疫激发应答中的作用。我们从纯合子（CC；n=2）与杂合子（C+；n=3）ChiaRed小鼠的肺部，分离得到野生型小鼠（WT；n=3）的肺上皮细胞，以及表达AMCase的ChiaRed阳性细胞，并通过RNA测序（RNA-Seq）分析比较上述细胞间的差异表达转录本。