Characterisation of TET function in drosophila larval central nervous system

Enzymes of the Ten Eleven Translocation (TET) family play a key role in the regulation of gene expression in many species by oxidizing 5-methylcytosine (5mC), a prominent epigenetic mark, into 5-hydroxymethylcytosine (5hmC). Yet, TET proteins also have non-canonical modes of action beyond 5mC oxidation, notably in Drosophila, whose genomes is devoid of 5mC. Here, we used a combination of NGS analyses to studied the funciton and mode of action of Tet in the central nervous system of Drosophila larvae. Overall design: All experiments were performed with biological replicates (duplicates for ChIP-seq and CUT&RUN; quadruplicates for RNA-seq). w1118 (wt)were used as control lines.