Interleukin-10 expression in the mouse spleen in the LPS-induced model

In the LPS-induced innate immune response, the expression level of IL-10 in the spleen of mice was significantly increased. The expression of this key anti-inflammatory cytokine is regulated by local sympathetic projections; however, the cell type in which it is expressed is unknown. Therefore, in this study, we used the LPS-induced mouse spleen model to analyze the spatial transcriptome, and found that IL10+ cells were mainly B cells. In addition, single-cell sequencing analysis of B cells isolated from the spleen of mice also showed that they expressed IL10. These results collectively suggest that B cells are the predominant cell type expressing IL10 in mouse spleen. Wild-type mice were injected with LPS via tail vein to induce innate immune response. Four hours later, the spleens of mice were collected, and CD45+ CD3-CD19 + B220+ B cells were sorted for single cell sequencing analysis.