Genetic network profiles associated with established resistance to ionizing radiation in leukemia cell line HL60

Radiation-resistant of acute myeloid leukemia cell present challenges to treatment, and the acquisition of resistance to ionizing radiation (IR) is a matter of clinical concern. However, little information is available on the behavior of radio-resistant leukemia in terms of gene expression profiles and intercellular communication. In this study, mRNA microarray was used to analyze the intracellular genetic network in the established radio-resistant HL60 (Res-HL60) cell line. Significant changes in the expression of 7,309 known mRNAs were observed in Res-HL60 relative to control. In addition, 7 mRNAs were determined as targets because significant changes in the expression were observed using Ingenuity analysis software, confirming the quantitative RT-PCR. However, extracellular vesicles from Res-HL60 cells did not include these target mRNAs. These results suggest that radio-resistant leukemia model is regulated by the expression and suppression of specific mRNAs, and these molecules are not transferred between cells by extracellular vesicles. Human acute myeloid leukemia cell line HL60 was prepared after frequent irradiation (4Gy X-irradiation ×4 fractions per 4 weeks) with confirmed radiation-resistance characteristics. The extracted total RNA in wild type cell and radiation-resistant cell were performed messenger RNA (mRNA) microarray and calculate the expression of mRNAs.