Comparison of value ranges describing particular intronic parameters in the test set of G⁺¹ mutations.

The BS and PPT parameters were predicted using prediction programs provided by Kol et al. [10] or Schwartz et al. [11] using the Sroogle engine. Computational predictions and other sequence parameters for an experimentally confirmed test set of splicing-affecting and non-affecting G+1 mutations were subjected to statistical analysis using the Mann-Whitney test (see Table S1 File S1 for details). Significant differences (p<0.05) are marked in bold. dist. = distance, BS = branch point site, M-W test = Mann-Whitney test, perc. = percentile, PPS = the longest uninterrupted polypyrimidine stretch Py = number of pyrimidines (in 25 or 50 nt upstream from 3′ss).