Effect of RPD3 phosphorylation on gene expression in glucose and sucrose

Effect of RPD3 phosphorylation on gene expression in glucose and sucrose Overall design: Total RNA was isolated from exponential growing yeast cells by standard phenol-chloroform extraction procedures and the quality of RNA was examined using Agilent Bioanalyzer according to the manufacturer's instructions. Library construction, sequencing and bioinformatics analysis were performed . There are three biological replicates for WT and Rpd3-2SA mutants in glucose and sucrose treatment conditions. Differential expression levels of aligned sequences were calculated using significant thresholds set at fold change over 1.5 and adjusted P-value < 0.05