Mobility of condensin DC is key to its function in repressing transcription [ChIP-seq]. Mobility of condensin DC is key to its function in repressing transcription [ChIP-seq]

Analyze condensin binding by fluorescence recovery after photobleaching (FRAP) and chromatin immunoprecipitation followed by sequencing (ChIP-seq) in ATP hydrolysis mutant and in mutants that affect the level of histone H4K20 methylation and H4K16ac enrichment and depletion on the X chromosome. We have also performed Hi-C analysis to test how 3D DNA interactions mediated by condensin DC change in the catalytic and null mutant of dpy-21, a H4K20me2 demethylase that increase H4K20me1 on the X. Overall design: Population ChIP-seq profiles of mixed embryo worms harboring rex site insertions and a dCas9-SunTag block strain