Pigment epithelium-derived factor (PEDF) on gene expression of mouse empbryonic fibroblasts (MEF)

Exercise training has been reported to induce changes in cellular homeostasis in various tissues, but the exact mechanisms remain unclear. Our findings demonstrate that in a mouse model, muscle-derived PEDFs have the capacity to intricately regulate cellular function at distal sites after training. The aim of this study was to investigate the effects of PEDF on gene expression in cultured MEFs and to elucidate the complex mechanisms involved. To investigate the effects of PEDF on gene expression, wild-type MEF at passage 2 were treated or untreated with PEDF (100 ng/mL) for 24h, and RNA expression was analyzed by RNA-seq. Two independent experiments were conducted. In each experiment, MEFs were prepared from different embryos.