A dopamine-induced coordinated gene expression program regulates neuronal function and cocaine response

The goal of this experiment was to determine how dopamine receptor activation in medium spiny neurons alters rapid transcriptional programs. To identify genes altered by dopamine, we used a rat primary striatal neuronal culture system in which striatal neurons were removed from the rat brain at embryonic day 18, cultured for 11 days in vitro using standard neuronal conditions, and treated neurons with either vehicle (neurobasal media) or dopamine (at a concentration of 1µM) for 1hr. This dataset contains PolyA+ RNA-seq results from this experiment, in which we identified 100 upregulated genes and 3 downregulated genes after dopamine stimulation using DESeq2 pipelines.本实验旨在探究中等多棘神经元（medium spiny neurons）内的多巴胺受体激活如何调控快速转录程序。为鉴定受多巴胺调控的差异表达基因，本研究采用大鼠原代纹状体神经元培养体系：于胚胎第18天从大鼠脑组织中分离纹状体神经元，经标准神经元培养条件体外培养11天后，分别以溶剂对照（Neurobasal培养基）或1μM多巴胺处理神经元1小时。本数据集包含本实验的polyA+ RNA测序（PolyA+ RNA-seq）结果，通过DESeq2分析流程鉴定得到，多巴胺刺激后共计100个上调基因与3个下调基因。