Ip, Wallace (2010) CIL:6257, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6265.

在5 mM MgATP的存在下，将兔子腰大肌肌纤维进行混合，以分离粗细肌丝。随后，以0.3M KCl温和替换介质，进一步分离粗肌丝。分离后的肌丝被吸附于云母片上（Heuser JE，J Mol Biol. 1983 Sep 5;169(1):155-95），并在Heuser型冷冻压机中通过接触液氦冷却的铜块进行快速冷冻。随后，在Balzers 400冷冻断裂机中进行冷冻蚀刻。样品随后以11度的Pt-C进行旋转复制，并在运行于100 kev的JEOL 100CX2透射电子显微镜下进行可视化。该图像使用柯达4489电影胶片以75,000倍放大率记录。在+5度和-5度处拍摄了立体对。胶片使用尼康Coolscan 9000ED扫描仪进行扫描，像素间距为20微米。与CIL:6265对应的立体对。