Raw data supporting metabarcoding unsorted kick-samples research

Raw data supporting: Metabarcoding unsorted kick-samples facilitates macroinvertebrate-based bioassessment with increased taxonomic resolution, while outperforming environmental DNA. Two-step PCRs were performed on each sample replicate using the Qiagen Multiplex PCR Plus Kit. The pool was loaded onto an Illumina MiSeq at 9pM, with 5% Phi-X, using a 600 cycle V3 kit with 300 bp paired end sequencing (index read steps skipped).<br> <br> Also available in the NCBI SRA: https://www.ncbi.nlm.nih.gov/sra/PRJNA629361