The network Regulation of Yiqi Jiedu Formulae against Cerebral Ischemia Based on Central-periphery Inflammation System

Connections between inflammation and gene-network regulation are suggested important in understanding the therapeutic target of stroke and in illuminating underlying mechanism. However, studies on the establishment of network relating with inflammation during stroke process are still in their early stages. We selected the inflammation genes from the whole mRNA expression results. And after a series of analysis, we found the promising target genes of Yiqi Jiedu formulae used in clinic for stroke, which might give an explanation to the common characteristic about TCM treatment called PK-PD inconsistent. In this study, we chosen 6 h after MCAO operation. The rats were divided into 3 groups: 6h sham group, 6h vehicle control group, 6h YJ groups (25mg•kg−1). After MCAO operation, the animals were sacrificed to obtain brain, spleen and blood respectively. RNAs of the three parts of each rat were extracted with Qiagen RNA kit. Total RNA was reverse transcribed, amplified, labelled and hybridized to Rat Genome 1.0 arrays (Affymetrix).