mRNA levels changes after OHT addition in SH-SY5Y cells.

RT2Profiler human oxidative stress and antioxidant defense PCR Arrays (Bioscience) were performed according to the manufacture’s protocols. Expression levels were compared between with and without OHT addition. Hypoxanthine phosphoribosyltransferase 1 gene was used as control for each gene expression calculation, and the extent of change in the expression of each gene was calculated by the ΔCt method. Only genes whose expression was downregulated at least 2 fold are shown in the Table. We have also removed all genes whose expression change significantly between duplicates. When ΔCt was over 12 and therefore expression was thought to be extremely low, the gene was omitted from analysis.