Quantification of Human Kallikrein‑2 in Clinical Samples by Selected Reaction Monitoring
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https://figshare.com/articles/dataset/Quantification_of_Human_Kallikrein_2_in_Clinical_Samples_by_Selected_Reaction_Monitoring/2370682
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资源简介：
Recently, the number of mass spectrometry-based
quantification
assays has been increased, partially due to the global efforts of
chromosome-centric human proteome project (C-HPP). Our goal at the
Chromosome 19 Consortium is to provide novel selected reaction monitoring
(SRM) assays of proteins coded on chromosome 19. We have selected
the two most useful signature peptides (NSQVWLGR and HNLFEPEDTGQR)
of human kallikrein-2 (hK2 – NX_P20151) and developed an SRM
assay. Details of the analytical parameters, including multiple transitions
by peptides, are presented. The endogenous levels of hK2 were determined
in clinical samples (n = 35). The limit of quantification
was also estimated by spiking heavy isotope-labeled peptides into
seminal plasma samples at various concentrations (LOQ ≈ 29
ng/mL).
创建时间：
2013-10-04
