LC-MS/MS analysis of protein samples obtained from native PAGE.
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Protein samples were cut out of all BSH-active bands from native polyacrylamide gel (Figure 3). Sample origin: 1, B. adolescentis DSM 20087; 2, B. animalis subps. animalis NRRL B-41406; 3, B. animalis subps. lactis NRRL B-41405; 4.1, 4.2; B. bifidum DSM 20456; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7, B. catenulatum DSM 20224; 8, B. infantis ATCC 15697; 9, B. longum NRRL B-41409; 10, B. pseudocatenulatum DSM 20439; 11.1, 11.2, 11.3; B. pseudolongum DSM 20099; 12, B. suis NRRL B-41407.
aProtein scores derived from individual ion scores of Mascot-identified tryptic peptides as a non-probabilistic basis for ranking protein hits.
bTotal number of tryptic peptides that the Mascot program assigned to a database protein. To compute this number, multiple matches to bifidobacterial peptides with the same primary sequence but representing different charge or modification states were counted as one.
LC-MS/MS analysis of protein samples obtained from native PAGE.
创建时间：
2014-12-03
