Full-length circRNAs in IMR-90 and A549 cell lines using nanopore sequencing
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https://www.ncbi.nlm.nih.gov/sra/SRP497610
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To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing. Overall design: Full-length circRNAs in IMR-90 and A549 was identified using nanopore sequencing
创建时间:
2025-06-26



