Full-length circRNAs in IMR-90 and A549 cell lines using nanopore sequencing

To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing. Overall design: Full-length circRNAs in IMR-90 and A549 was identified using nanopore sequencing