Raw sequence data from three EMS-treated populations and one control population of Saccharomyces cerevisiae (BY4741, derived from the S288C strain)

The goal of this study was to investigate how a chemical called EMS (Ethyl methanesulfonate) introduces mutations in the DNA of yeast cells (Saccharomyces cerevisiae) and to compare the mutation numbers and types with an untreated control group. EMS is known to cause mutations by altering the genetic material, and we wanted to see how many and what kinds of changes it would cause in yeast.To conduct the experiment, we first grew a population of yeast cells in a liquid culture until they reached a specific density (OD596 = 0.1), which indicates the number of cells present. We then added EMS to the culture at a concentration of 10 ug/ml and incubated it at 30 degrees Celsius for 1 hour, shaking the culture at 250 rpm to ensure even exposure to the EMS.After the incubation, it was essential to stop the EMS from continuing to damage the cells, so we washed the cells twice with a chemical called sodium thiosulfate, which neutralizes the EMS. We followed this by washing the cells with water to remove any remaining traces of EMS. These treated cells were then plated, and 5 strains per population were selected and compared with untreated control cells to measure the number and types of mutations that occurred as a result of the EMS exposure. The populations came from independent biological replicates.This study provides insight into how EMS treatment alters the yeast genome and gives insight into the type and number of mutations introduced via EMS treatment.