M. Huranova, I. Ivani, A. Benda, I. Poser, Y. Brody, M. Hof, Y. Shav-Tal, K.M. Neugebauer, D. Stanek (2011) CIL:26583, Homo sapiens, cervical epithelium. CIL. Dataset
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HeLa cells stably expressing BAC-encoded GFP-labeled snRNP protein hPrp31 and imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. The hPrp31 is confined to the nucleoplasm and enriched in splicing factor compartments and Cajal Bodies (bright dots). From Fig 1A in Huranova et al. 2010 J Cell Biol 191:75-86.
HeLa细胞稳定表达BAC编码的GFP标记的snRNP蛋白hPrp31,并使用Leica SP5共聚焦显微镜配备60倍1.4NA HCX Plan Apo物镜进行成像。hPrp31蛋白局限于核质,并在剪接因子区室及Cajal小体(明亮的斑点)中富集。详见Huranova等人于2010年发表在《细胞生物学杂志》(J Cell Biol)第191卷第75-86页的图1A。
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